On March 14, 1883, when Prague botanist Friedrich Reinitzer heated cholesterylbenzoate, he observed the abnormal behavior of benzoic acid cholesterol ester during hot melting: it melted at 145.5 ° C, producing a cloudy substance with a bright color. As the temperature increased, the luster disappeared and the liquid became transparent. When the compound cools down, the same phenomenon repeats, but the order is reversed, ultimately forming a solid crystal. The temperature at which it melts is called the melting point. The temperature at which the liquid crystal becomes transparent is called the clearing point of the liquid crystal.
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