Explanation

It refers to the ability of certain factors (chemical, physical, and biological) to cause cell lysis (cell death), inhibit cell growth, or produce other adverse reactions to cells. The detection is mainly based on changes in cell membrane permeability, and the following three methods are commonly used: thiazolium (MTT) method, XTT, and lactate dehydrogenase (LDH) method. There are three main methods for qualitative evaluation of biological materials. ① The direct contact method is applicable to materials with low density, such as contact lenses. The test materials is directly placed on the cell line, and then heated at an appropriate temperature to promote the diffusion of soluble chemicals in the materials to the culture medium and contact the cells. ② Agar diffusion method is applicable to materials with high density, such as artificial rubber plugs. Cover a piece of nutrient supplemented agar on the cultured cell line, and then put the test materials on the agar layer. Dissolved chemicals in materials will diffusion to agar and contact with cells. ③ The minimum basic medium (MEM) elution method, which is used to judge the materials extract, usually simulates the actual use state or the worst case, and extracts with different extractants and different conditions. Then transfer the extract to the cell layer and heat it, and observe under a microscope whether the cells are deformed, degraded, and decomposed. The above three methods can be used for qualitative analysis of medical materials.

Classification